Research Subject

Development towards multi-omics single cell analysis platform

Yasushi Okada

RIKEN Quantitative Biology Center
Cell Dynamics Research Core Laboratory for Cell Polarity Regulation
Team Leader

Quantitative Biology Center (QBiC), RIKEN, 6-2-3, Furuedai, Suita, Osaka 565-0874
TEL:06-6155-0118 MAIL:y.okada@riken.jp

Fluorescent live cell imaging has proven the importance of the single cell analysis. Recent technical advancements including super-resolution microscopy further increase its importance. However, it is limited in throughput and difficult to use for comprehensive analysis such as omics studies. Among various omics technologies, DNA-sequence based omics methods are now being applied for single cell analysis, while proteomics or metabolomics, both based on mass spectrometry, are difficult to apply to single cell analysis. In this project, we strengthen two original technologies of QBiC: super-resolution fluorescent live cell imaging and single cell mass spectroscopy. In addition to the improvement of these technologies, we will develop a platform for multi-omics single cell analysis by merging DNA-sequence based omics approaches with high resolution microscopy.

Spinnning disk super-resolution microscope for high spatio-temporal resolution live cell imaging.

Spinnning disk super-resolution microscope for high spatio-temporal resolution live cell imaging.

Live imaging of the outer membrane of mitochondria. Left: conventional image, right: super-resolution image

Live imaging of the outer membrane of mitochondria.
Left: conventional image, right: super-resolution image

Schematic principle of single cell metabolomics

Schematic principle of single cell metabolomics

Team Member