Fluorescent live cell imaging has proven the importance of the single cell analysis. Recent technical advancements including super-resolution microscopy further increase its importance. However, it is limited in throughput and difficult to use for comprehensive analysis such as omics studies. Among various omics technologies, DNA-sequence based omics methods are now being applied for single cell analysis, while proteomics or metabolomics, both based on mass spectrometry, are difficult to apply to single cell analysis. In this project, we strengthen two original technologies of QBiC: super-resolution fluorescent live cell imaging and single cell mass spectroscopy. In addition to the improvement of these technologies, we will develop a platform for multi-omics single cell analysis by merging DNA-sequence based omics approaches with high resolution microscopy.
Development towards multi-omics single cell analysis platform
RIKEN Quantitative Biology Center
Cell Dynamics Research Core Laboratory for Cell Polarity Regulation
Quantitative Biology Center (QBiC), RIKEN, 6-2-3, Furuedai, Suita, Osaka 565-0874