Research Subject

Highly Quantitative Subcellular Metabolomics by Capillary Electrophoresis with Mass Spectrometry (CE-MS)

Takayuki Kawai

RIKEN Quantitative Biology Center
Cell Design Research Core Laboratory for Synthetic Biology
Laboratory for Integrated Biodevice
Special Postdoctoral Researcher

Graduate School of Frontier Biosciences, Osaka University (BioSystems Building) 1-3 Yamadaoka, Suita, Osaka 565-0871

The goal of this project is to establish quantitative metabolome analysis of subcellular-size samples with high accuracy and precision. As the base technology, I will employ capillary electrophoresis (CE) coupled with mass spectrometry (MS). In CE-MS, we can separate numerous compounds including structural isomers that are difficult to analyze in normal single-cell MS analysis, so that we can avoid ion suppresion and can qualify/quantify metabolites with high sensitivity. I will fabricate tapered short capillary, with which a subcellular sample (fL-pL) will be collected via micromanipulation with good spatial resolution. The tapered structure will also work for efficient ionization that leads to ultra-sensitive quantitation. In the future, this technology will be usefull in research area like neuroscience, i.e., analyzing where and how metaolites function in subcellular-scale dimension.

Capillary electrophoresis-mass spectrometry (CE-MS) instrument.

Capillary electrophoresis-mass spectrometry (CE-MS) instrument.