Research Subject

Direct and controllable organelle transfer between live single cells by using micro-fabricated device

Ken-Ichi Wada

RIKEN Bioengineering Laboratory
Contract Researcher

RIKEN Bioengineering laboratory, 2-1 Hirosawa, Wako-shi, Saitama 351-0198,
TEL:048-467-9312 MAIL:ki-wada@riken.jp

Cellular phenotype differs from each other even in same population, namely, cells have their own individuality. The mechanism for arising of cellular individuality is an important target of single-cell research. Because organelles have great involvements with controlling cellular behaviors, this research project aims to develop a novel single-cell manipulation technique which contributes to investigation of cellular individuality focusing on organelles. In particular, a novel cell fusion method using micro-fabricated device, in which cells are fused through a micro aperture, leading to direct transferring of organelle(s) under quantitative control, will be developed. This novel single-cell manipulation technique will provide an unique and useful experimental platform for single-cell researches.

Organelle transfer system using micro-fabricated device. This device can perform direct organelle transfer via cell fusion through a microslit.

Organelle transfer system using micro-fabricated device. This device can perform direct organelle transfer via cell fusion through a microslit.

Examples of direct organelle transfer. This figure shows a typical example of direct transfer of peroxisomes.

Examples of direct organelle transfer. This figure shows a typical example of direct transfer of peroxisomes.

Controlled organelle transfer. This research project aims to develop a novel single-cell manipulation technology using microdevice, which can perform direct organelle transfer with qualitative and/or quantitative controls.

Controlled organelle transfer. This research project aims to develop a novel single-cell manipulation technology using microdevice, which can perform direct organelle transfer with qualitative and/or quantitative controls.