Cellular phenotype differs from each other even in same population, namely, cells have their own individuality. The mechanism for arising of cellular individuality is an important target of single-cell research. Because organelles have great involvements with controlling cellular behaviors, this research project aims to develop a novel single-cell manipulation technique which contributes to investigation of cellular individuality focusing on organelles. In particular, a novel cell fusion method using micro-fabricated device, in which cells are fused through a micro aperture, leading to direct transferring of organelle(s) under quantitative control, will be developed. This novel single-cell manipulation technique will provide an unique and useful experimental platform for single-cell researches.
Direct and controllable organelle transfer between live single cells by using micro-fabricated device
RIKEN Bioengineering Laboratory
RIKEN Bioengineering laboratory, 2-1 Hirosawa, Wako-shi, Saitama 351-0198,