I wish to adapt a single cell screening method established by Dr. Jay Shin in the Center for Life Science Technologies (CLST) in RIKEN Yokohama. This single cell screening method will be combined with CRISPR interference (CRISPRi) to perturb different sub-types of induced neurons derived by the overexpression of a defined set of neuronal transcription factors in hiPSC.
Inferring molecular mechanisms of neural reprogramming and maturation using single cell analysis
RIKEN Center for Life Science Technologies
Division of Genomic Technologies Cell Conversion Technology Unit
1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan